In this tutorial, we will cover MTT assays. MTT assays are quick, simple, and cheap assays that can be used to estimate proliferation and viability in cells under a multitude of conditions such as drug treatments, time, gene knockouts, etc. As such, they are a powerful tool in any lab. The videos in this tutorial will walk you through the theory, practice, and analysis of this assay. In addition, this tutorial contains a list of the reagents necessary to perform this experiment along with written out protocols, planning and analysis templates, and real-world examples.