Antibodies are widely used in scientific applications these days. They are vital for a variety of bench science experiences, including immunohistochemistry, immunostaining, immunoprecipitation, western blots, flow cytometry, ELISAs, and so much more. However, it's often hard to understand how to choose antibodies and use them together for colocalization stains when you start out. This tutorial is directed at beginners and goes over multiple examples to explain exactly how to choose and use these antibodies together for molecular biology applications. We provide multiple examples in the resources section for your reference.